Serum amyloid A (SAA)

Anti-SAA antibody (Datasheet)

Description	Mouse monoclonal antibody
Catalog number	K7c5	K8d6	K9f7	K11h8
Applications	Detection	Capture	Reagent 2	Reagent 2
Platforms	Immunofluorescence, Colloidal gold		Immunological latex turbidimetry
Buffer	1 x PBS
Purity	Purity>98%，purified by Protein A/G chromatography
Storage instructions	Upon delivery aliquot and store at -80°C. Avoid freeze / thaw cycles.

SAA protein (Datasheet)

Catalog number	K1936
Applications	Calibrator and quality control product
Source	E. coli
Purity	>90% as analysized by SDS-PAGE
Buffer	1 x PBS，pH 7.4
Storage instructions	Upon delivery aliquot and store at 2-8°C. Avoid freeze / thaw cycles.
Western-Blot

Antibody Product Information

Antibody Evaluation (Immunofluorescence-based)

Immunofluorescence-Based test strip was prepared with K7c5-K8d6 matched antibody pairs (30 Siemens calibrators, Concentration: 3-300 ug/ml, R² > 98%)

Fig. 1 Coincidence rate of Immunofluorescence-based test strip (K7c5-K8d6)

Antibody Evaluation (Immunological Turbidimetry)

Fig. 2 Calibration curve

R1: Buffer

R2: Combination of K1016-K1017 matched antibody pairs and latex particals

Sample: SAA calibrator

Equipment: Hitachi 7080

Linear range

Fig. 3 Linearity range

Theoretical value(ng/ml)	Measured value 1	Measured value 2	Average
2.05	2.22	2.01	2.115
33.70	32.22	32.02	32.12
65.36	65.8	62.8	64.3
128.67	118.25	127.36	122.805
255.29	248.76	256.74	252.75
508.52	512.36	509.08	510.72

Table 1. Sample concentrations

Six calibrators were prepared using Latex particals and SAA antibody. Data show that Linear dependence > 99% with concentration between 2-500 ug/mL as indicated in Fig. 3 and Table 1.

Clinical sample analysis (Immunological Turbidimetry-based)

SAA antibody was employed to prepare kits to detect 48 clinical coincidence rates. With R² > 99%, the antibody can be used to develop SAA immunonephelometry materials.

Fig. 4 Clinical sample analysis

Clinical sample analysis (Colloidal Gold-based)

Fig. 5 Coincidence rate of colloidal gold-based test strip (K7c5-K8d6)

The strip was prepared with K7c5-K8d6 matched antibody pairs. With R2 > 99% of 48 Siemens clinical samples, the antibodies can be used to develop SAA immunonephelometry materials.

Accuracy

The quality control samples with concentration of 10 ng/ml-20 ng/ml and 70 ng/ml-80 ng/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)

	Sample(μg/ml)	Test 1	Test 2	Test 3	Average(μg/ml)	RSD	Standard
Batch 1	15.60	15.76	16.22	15.15	15.71	0.71%	±15%
Batch 1	78.00	80.22	85.52	75.58	80.44	3.13%
Batch 2	15.60	14.85	15.94	16.17	15.65	0.34%
Batch 2	78.00	76.92	75.81	84.54	79.09	1.40%
Batch 3	15.60	16.55	16.01	15.49	16.02	2.67%
Batch 3	78.00	79.47	82.33	78.44	80.08	2.67%

Repeatability

The quality control samples with concentration of 10 ug/ml-20 ug/ml and 70 ug/ml-80 ug/ml respectively were required to repeat test over 10 timers. CV= (S / Average

) ×100% (CV < 15% as indicated in the following table)

	Low Value (15.60 ng/ml)	High Value (78.00 ng/ml)
Test 1	11.19	54.70
Test 2	12.01	50.21
Test 3	11.32	53.54
Test 4	12.53	56.01
Test 5	11.47	49.27
Test 6	10.93	54.80
Test 7	10.88	50.70
Test 8	11.89	47.16
Test 9	11.05	47.39
Test 10	12.41	48.35
Average(μg/ml)	11.57	51.21
SD	0.61	3.30
CV	5.23%	6.43%
Standard	±15%

Description

Serum amyloid A (SAA) is an AP apolipoprotein associated with high-density lipoproteins (HDL) in plasma. It is reported that SAA as a biomarker in a variety of diseases ranging from bacterial infection, virus infection, atherosclerosis, coronary heart disease, acute rejection of transplantation and cancer. SAA showed a wider range of concentrations and a significantly superior overall diagnostic performance compared with CRP in diagnosis of certain diseases.

SAA is also a strong marker for cardiovascular and all-cause mortality, interacting with the functionality of HDL. Concomitantly measuring SAA concentrations may represent a convenient method to estimate HDL functionality in clinical routine.

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Serum amyloid A (SAA)

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